Staff Marcin BALCERZAK, Cezary BREGIER(PhD student),
Hanna FABCZAK,
Wojciech GRAJKOWSKI(PhD student),
Bożena GROSZYŃSKA(technician),
Piotr KOPROWSKI,
Andrzej KUBALSKI,
Leszek KUŹNICKI(Professor Emeritus),
Anna MIRGOS (technician),
Katarzyna SOBIERAJSKA(PhD student),
Anna WASIK
Research profile
Physiological role of phosducin (Phd) and phosducin-like (PhLPs)
proteins as regulators of G-protein trunsduction pathway
and/or modulators of chaperonin CCT function
during folding of cytoskeletal proteins
(tubulin, actin) and microtubule assembly in selected
ciliates (Blepharisma, Paramecium, Stentor,
Tetrahymena). Patch-clamp electrophysiological
investigations characterizing the structure and function of
mechanosensitive ion channels in Escherichia coli.
The role of extracellular factors
in dynamics of actin
cytoskeleton in Amoeba proteus
with the identification and determination of the
function of actin binding proteins.
Current research activities
studies of the PhLPs function as chaperonin CCT modulator
during tubulin folding;
elucidation of the consequence of PhLPs gene expression
silencing on microtubule assembly, cilia function and
ciliate movement parameters;
patch-clamp studies on mechanosensitive ion channels from
the cytoplasmic membrane of Escherichia coli and
investigation of the role of their C-termini in the channel
gating;
identification of actin binding proteins responsible for
actin cytoskeleton reorganization in Amoeba proteus;
role of extracellular factors on
dynamics of actin
cytoskeleton in Amoeba proteus.
Selected publications
Koprowski P., Grajkowski W., Kubalski A. (2007) The MscS cytoplasmic domain and its conformational changes on the channel gating. Curr. Topics in Membranes, vol. 58. Mechanosensitive ion channels. Part A. Owen P. Hamill, ed., 295-309.
Pomorski P., Krzemiński. P., Wasik A., Wierzbicka K., Barańska J., Kłopocka W. (2007) Actin dynamics in Amoeba proteus motility. Protoplasma231: 31-41.
Sobierajska K., Fabczak H, Fabczak S. (2007) Phosducin interacts with the G-protein βγ-dimer of ciliate protozoan Blepharisma japonicum upon illumination. J. Exp.Biol. 210: 4213-4223.
Fabczak H., Fabczak S. (2006) Photosensory transduction in unicellular eukaryotes. A comparison between ciliate protists and photoreceptor cells of higher organisms (Invited review). J.Photochem. Photobiol. 83: 163-171.
Grajkowski W., Kubalski A., Koprowski P. (2005) Surface changes of the mechanosensitive channel MscS upon its activation, inactivation and closing. Biophys. J. 88: 3050-3059.
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