Laboratory of Molecular Basis of Cell Motility
Head
Maria Jolanta RĘDOWICZ
Staff: Jolanta Jóźwiak, Justyna Karolczak (PhD student), Łukasz Majewski (PhD student), Hanna Nieznańska, Krzysztof Nieznański, Iuliia PAVLYK (PhD student), Paweł Pomorski, Dariusz Stępkowski, Dorota Wypych, Tomasz Zajkowski (PhD student)
Research profile:
• involvement of unconventional myosins and actin-binding proteins in cell migration and intracellular trafficking – studies on normal and transformed mammalian cells, and amoebae
• role of myosin VI in myogenesis and skeletal muscle functioning
• plasticity of the contractile apparatus from skeletal muscles
• role of prion protein-induced oligomerization of tubulin and phosphorylation of tau protein in the pathogenesis of prion diseases
• effects of arginine deprivation on actin cytoskeleton, cell migration and invasiveness
• formation and cytoskeletal modulation of the receptor- generated calcium signal in living cells
• regulation and mechanism of capacitative calcium signal
• role of calcium signaling in the regulation of cell motility
Methods:
• protein chemistry
• biochemistry
• fluorescence spectroscopy
• molecular and cell biology (micromanipulations, measurements of intracellular ions concentration, in vivo observations of cell motility and adhesion)
• fluorescence and electron microscopy
Current research activities:
• investigation of the involvement of class VI myosin in cell locomotion and intracellular trafficking
• studies on the function of amebin, a novel actin-binding protein from Amoeba proteus
• analysis of the contractile apparatus in normal and atrophied muscles
• probing the interaction of prion protein with microtubular cytoskeleton
• fast calcium signal measurements
• imaging of receptor-induced calcium signal in primary culture neurons
• crosstalk between calcium and RhoA signaling pathways
Selected publications:
Osiecka K.M., Nieznańska H., Skowronek K.J., Nieznański K. (2011) Tau inhibits tubulin oligomerization induced by prion protein. Biochimica et Biophysica Acta, 1813: 1845-1853.
Jóźwiak J., Rzhepetskyy Y., Sobczak M., Kocik E., Skórzewski R., Kłopocka W., Rędowicz M.J. (2011) Amebin, a novel Amoeba proteus actin-bundling protein inhibits actomyosin ATPase activity and is essential for amoeba migration. Archives of Biochemistry and Biophysics, 506: 99-108.
Havrylov S., Rędowicz M.J., Buchman V.L. (2010) Emerging roles of Ruk/CIN85 adaptor protein in vesicle-mediated transport, adhesion, migration and malignancy. Traffic, 11: 721-731.
Majewski L., Sobczak M., Rędowicz M.J. (2010) Myosin VI is associated with secretory granules and is present in nucleus in adrenal medulla chromaffin cells. Acta Biochimca Polonica, 57: 109-114.
Osiecka K.M., Nieznańska H., Skowronek K.J., Karolczak J., Nieznański K. (2009) Prion protein region 23-32 interacts with tubulin and inhibits microtubule assembly. Proteins, 77: 279-296.
