Laboratory of Confocal Microscopy
Head: Wanda KŁOPOCKA
Staff: Wojciech Brutkowski, Jarosław Korczyński, Aleksandra Ławrynowicz, Artur Wolny
The Laboratory of Confocal Microscopy was established in May 2002. This is an open laboratory available to all scientists from the Nencki Institute and other scientific institutions.
The laboratory is equipped with:
• two confocal scanning systems: Leica SP5 STED and Leica TCS SP2, both with multiphoton lasers. The appliances use HeNe 633 nm; GreNe 543 nm; Ar 458 nm, 476 nm, 488 nm, 514 nm and Mai Tai IR 710 nm-990 nm lasers as the source of light
• TCS SP5 STED covers a hi-resolution morphology system and Life Imaging techniques. STED allows spatial resolution down to 70 nm which gives possibility to explore precisely cell architecture. The resonant scanner of TCS SP5 enables to acquire high speed images at large format and to observe dynamic changes in a live cell. What is more, FRET and FRAP analyses are also available for users
• TCS SP2 system is equipped with objectives with long work distance and a heating chamber. The microscope is equipped with a unique FLIM (Fluorescence Lifetime Imaging) system used for molecules iteractions
• Zeiss TIRF microscope with HeNe 633 nm; GreNe 532 nm; Ar 488 nm lasers, 405 nm diode lasers and filters: double CFC/Cy3, triple GFP/mRFP/Aleca633, GFP. Microscope allows studies of processes in plasma membrane as well as microtubules and actin filaments dynamic
• Leica Epi-Fluorescence microscope DMI6000B coupled with b/w camera, set of fluorescence filters and Differential Interference Contrast (DIC) is also available
The laboratory was enriched with a new powerful graphic station and software: Huygens Professional.
The service in the laboratory may assist users in the field of image processing and analysis as well as the advice and assistance in use of the equipment.
More information about laboratory on the web page
Selected publications:
Geszke M., Murias M., Balan L., Medjahdi G., Korczyński J., Moritz M., Lulek J., Schneider R. (2011) Folic acid-conjugated core/shell ZnS:Mn/ZnS quantum dots as targeted probes for two photon fluorescence imaging of cancer cells. Acta Biomaterialia, 7: 1327-1338.
Słomnicki Ł.P., Leśniak W. (2010) S100A6 (calcyclin) deficiency induces senescence-like changes in cell cycle, morphology and functional characteristics of mouse NIH 3T3 fibroblasts. Journal of Cellular Biochemistry, 109: 576-584.
Kulma M., Hereć M., Grudziński W., Anderluh G., Gruszecki W.I., Kwiatkowska K., Sobota A. (2010) Sphingomyelin-rich domains are sites of lysenin oligomerization: implications for raft studies. Biochimica et Biophysica Acta, 1798: 471-481.
Nowicka D., Soulsby S., Skangiel-Kramska J., Głażewski S. (2009) Parvalbumin-containing neurons, perineuronal nets and experience-dependent plasticity in murine barrel cortex. European Journal of Neuroscience, 30: 2053-2063.
Karetko M., Skangiel-Kramska J. (2009) Diverse functions of perineuronal nets. Acta Neurobiologiae Experimentalis, 69: 564-577.
